Arachidonic Acid Content in the Feed on the Growth Performance, Antioxidant Capacity and Fatty Acid Generation of Sea Cucumber

Authors:Jerry King, Scott Smith

Abstract


In order to explore the effects of arachidonic acid (ARA) on the growth performance, antioxidant capacity and fatty acid metabolism of Apostichopus japonicus, the initial weight of (7.78±0.06) g was used as the research object. Using fishmeal and fermented soybean meal as the main protein source, wheat flour as the main sugar source to make the basic feed, by adding different proportions of ARA-purified oil to the basic feed, the ARA content was 0.02% (control group), 0.17%, The experimental feeds of 6 groups of nitrogen and other lipids of 0.36%, 0.51%, 0.59% and 0.98% (dry weight of feed) were subjected to a 56-day culture experiment in an indoor circulating aquaculture system. The results showed that with the increase of ARA content in feed, the weight gain rate (WGR) of the sea cucumber increased first and then decreased. The WGR of 0.36% and 0.51% ARA feed group was significantly higher than other treatment groups. (P<0.05), the specific growth rate (SGR) and feed efficiency (FE) of sea cucumber have the same trend as WGR; the crude fat content of sea cucumber body decreased first with the increase of feed ARA content. The increase trend was the lowest in the 0.51% ARA feed group, and significantly lower than the control group and the 0.98% ARA feed group (P<0.05). Meanwhile, with the increase of ARA content in the feed, the ARA and n in the wall of the sea cucumber -6 polyunsaturated fatty acids (n-6 PUFA) content increased significantly, while eicosapentaenioc acid (EPA), docosahexaenoic acid (DHA) And n-3 polyunsaturated fatty acids (n-3 PUFA) content decreased significantly (P<0.05); antioxidant capacity, 0.36% and 0.51% ARA feed group in the intestinal tract superoxide Superoxide dismutase (SOD), catalase (CAT) and total The activity of total antioxidant capacity enzyme (T-AOC) was significantly higher than that of the control group and 0.98% ARA diet group (P<0.05), while the content of malondialdehyde (MDA) in the intestine showed opposite trend. The activity of fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) in the intestinal tract of sea cucumber decreased significantly with the increase of ARA content (P<0.05). The activity of carnitine palmitoyltransferase-1 (CPT-1) in the intestinal tract of the sea cucumber increased first and then decreased with the increase of ARA content (P>0.05). Studies have shown that under the conditions of this experiment, the addition of proper amount of ARA (0.36%-0.51%) in the feed can promote the growth and antioxidant capacity of sea cucumber, and the results show that the content of feed ARA will be in the intestinal tract of sea cucumber. Fatty acid metabolism has a certain effect.


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